1/5/2024 0 Comments Amplifx primer list formatRabbit beta-globin intron, reverse primer Rabbit beta-globin intron, forward primer Psi packaging signal, 5' of MCS in pBABE vectors, forward primerģ' end of glutathione-S-transferase, forward primerįor Pichia vectors with AOX1 terminator, reverse primerįor Pichia vectors with AOX1 promoter, forward primerĭrosophila Actin 5C promoter, forward primerĪlpha factor signal sequence, forward primerĥ' end of ampicillin resistance gene, reverse primerįor Pichia vectors with AUG1 promoter, forward primerįor Pichia vectors with AUG1 promoter, reverse primerīovine growth hormone terminator, reverse primer Human CMV immediate early promoter, forward primer This list is available for your convenience.įor reference information, please consult Addgene's Molecular Biology Reference Page.Īll listed primers are 5′ to 3′. Below is a list of commonly used primers. Still not sure what primer you need? Email us at Īddgene has used a number of primers for sanger sequence verification of deposited plasmids. To identify primers that may be useful in your sequencing reaction, find your plasmid page and see what primers are listed under "5' sequencing primer" and "3' sequencing primer". Addgene does not distribute primers.įor sequencing plasmids in our repository, we've chosen primers based on the plasmid backbone and insert. The primer sequences listed on the left are provided for your reference.
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